A laser Raman spectroscopic study of Ca2+ binding to troponin C.
نویسندگان
چکیده
Laser Raman spectroscopy has been used detect structural changes in troponin C induced by Ca2+ binding. Addition of Ca2+ - Mg2+ sites produces perturbations in the amide III region of the spectrum indicative of increased alpha-helical content, and in regions of the spectrum corresponding to carboxylate, thiol, and phenol side chains. However, Ca2+ binding to the low affinity Ca2+ - specific sites is not detected by laser Raman spectral changes.
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ورودعنوان ژورنال:
- Biophysical journal
دوره 30 2 شماره
صفحات -
تاریخ انتشار 1980